* Gazprom says gas price for Ukraine to rise to $485 per 1,000 cubic metres
* Gazprom says Ukraine's gas debt stands at $2.2 billion
* Ukraine calls the price hike "political", refuses to pay
* Gazprom says Ukraine should refill gas storages for safe flows to Europe (Adds details, comments from Ukraine, analyst)
By Svetlana Burmistrova and Pavel Polityuk
MOSCOW/KIEV, April 3 (Reuters) - Russia increased the gas price for Ukraine for the second time in just three days to push prices up by 80 percent, piling the pressure on an economy already on the brink of bankruptcy.
Ukraine, which has a new International Monetary Fund loan package to cushion some of the blow, dismissed the increase as politically motivated, saying the price was too high and that it should not have to pay for supplies at this level.
Moscow has frequently used energy as a political weapon in dealing with its neighbours, and European customers are concerned Russia might again cut off deliveries in the worst East-West stand-off since the Cold War over Crimea.
The head of Russia's top natural gas producer, Gazprom , Alexei Miller told Prime Minister Dmitry Medvedev on Thursday that the price increase was due to the introduction of an export duty on gas.
"The gas price is increasing automatically from April," Miller said.
The latest rise will be to $485 per 1,000 cubic metres - two days after Gazprom announced a 44 percent hike in the gas price to $385.5 per 1,000 cubic metres from $268.5 due to unpaid bills.
Ukraine, which covers 50 percent of its gas needs with Russian supplies, condemned the move as politically motivated.
"The Ukrainian economy should not pay such a price for gas. It is a political price," Ukrainian Energy Minister Yuri Prodan told reporters in Kiev.
Valery Nesterov, an analyst with Sberbank CIB, said Ukraine could apply to an international court for a settlement.
Gazprom has had to agree to cut gas prices and improve contractual terms for its European clients, including those who successfully challenged the Russian company in courts.
The European Union receives around half of Russian gas via Ukraine.
"It would be extremely difficult for Ukraine to pay such a price. I think this is just an instrument for further negotiations used by Gazprom," Nesterov said.
Russia has moved to squeeze Ukraine's faltering economy after protesters toppled pro-Moscow President Viktor Yanukovich, installing new leaders bent on pursuing closer ties with the European Union.
Russia's annexation of Ukraine's Crimea region last month deepened tensions and Ukraine has vowed to take Russia to court over the seizure of Ukrainian assets there.
In hiking the price, Russia has scrapped two discounts simultaneously.
One was introduced in 2010 when Ukraine agreed to extend terms for Russia's Black Sea Fleet in Crimea until 2042, and the second was agreed in December after Yanukovich scrapped a trade deal with the European Union in favour of closer ties to Russia.
As part of that deal, the Russian government agreed to scrap gas export duties for Ukraine-bound gas.
Earlier this week, the Russian Federation Council, the upper house of the parliament, voted to annul the agreement on the Black Sea Fleet after Crimea was annexed by Russia.
On Thursday, Gazprom also said Ukraine had to increase the level of gas in storage to ensure its stable transit to Europe.
According to Ukraine's Energy Ministry the country holds 7.2 billion cubic metres in gas storage. It needs 12-14 billion cubic metres to ensure a stable flow of gas to Europe in winter. (writing by Vladimir Soldatkin, editing by Elizabeth Piper and William Hardy)